Preparation and application of alcohol based deep eutectic solvents for extraction of curcumin in food samples prior to its spectrophotometric determination

Altunay N., Elik A., Gürkan R.

FOOD CHEMISTRY, cilt.310, 2020 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 310
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1016/j.foodchem.2019.125933
  • Dergi Adı: FOOD CHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Aerospace Database, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Chimica, Communication Abstracts, Compendex, EMBASE, Food Science & Technology Abstracts, MEDLINE, Metadex, Veterinary Science Database, Civil Engineering Abstracts
  • Anahtar Kelimeler: Food samples, Alcohol based-deep eutectic solvent, Curcumin, Spectrophotometry, Microextraction, LIQUID-LIQUID MICROEXTRACTION, VOLTAMMETRIC DETERMINATION, TRACE CURCUMIN, RAT PLASMA, LC-MS/MS, WATER, PRECONCENTRATION, STABILITY, ACID, METHODOLOGY
  • Sivas Cumhuriyet Üniversitesi Adresli: Evet

Özet

In this study, a novel, simple and economic vortex-assisted alcohol-based deep eutectic solvent microextraction (VA-DES-ME) procedure has been developed for the preconcentration of curcumin in food samples prior to its spectrophotometric determination. A alcohol-based-DES consists of betaine hydrochloride - glycerol (1:3) as extraction solvent and acetone as aprotic solvent at pH 6.0 were used for the preconcentration of curcumin from sample matrix. Some experimental variables such as pH, DES solvent type and volume, aprotic solvent type and volume, vortex time, sample volume and matrix effect etc. were investigated and optimized in detail. Under optimized conditions, the working range, limit of detection and pre-concentration factor were determined to be as 5-300 mu g L-1 and 1.5 mu g L-1 and 100 respectively. Validity of the proposed method was proved by the intraday/inter-day precision studies and addition-recovery test. Consequently, the VA-DES-ME procedure was successfully applied for the extraction and determination of curcumin in food samples.