Optimization of an ultrasound-assisted alcohol-based deep eutectic solvent dispersive liquid-phase microextraction for separation and preconcentration of quercetin in wine and food samples with response surface methodology

ALTUNAY N., ELİK A., ÜNAL Y., KAYA S.

JOURNAL OF SEPARATION SCIENCE, cilt.44, sa.9, ss.2006-2014, 2021 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 44 Sayı: 9
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1002/jssc.202100048
  • Dergi Adı: JOURNAL OF SEPARATION SCIENCE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Aerospace Database, BIOSIS, CAB Abstracts, Chemical Abstracts Core, Chimica, Communication Abstracts, Compendex, EMBASE, Food Science & Technology Abstracts, INSPEC, MEDLINE, Metadex, Veterinary Science Database, Civil Engineering Abstracts
  • Sayfa Sayıları: ss.2006-2014
  • Anahtar Kelimeler: alcohol&#8208, based deep eutectic solvents, multivariate optimization, quercetin, real samples, spectrophotometry
  • Sivas Cumhuriyet Üniversitesi Adresli: Evet

Özet

We optimized ultrasound-assisted alcohol-based deep eutectic solvent dispersive liquid-phase microextraction for separation and preconcentration of quercetin in wine and food samples by experimental design based on central composite design. Five different alcohol-based deep eutectic solvents were prepared and tested for quercetin extraction. The effect of important parameters and matrix components were optimized. After optimization, the determination of quercetin was performed at 385 nm using spectrophotometry. Analytical data such as detection limit, working range and preconcentration factor were found as 6.1 mu g/L, 20-850 mu g/L, and 120, respectively. The selectivity of the optimized extraction conditions for quercetin was investigated in the presence of different matrices. The validation of the method was investigated by reproducibility, repeatability and recovery studies, as well as by comparing the analytical results obtained from real samples with the reference method. Lastly, the recommended procedure was successfully applied for the extraction and quantification of quercetin in wine and food samples.