Chemical and biological characterization of sulfated chitosan oligomer as heparin mimics

Kocabay S., Bahar M. R., TEKİN S., AKKAYA R., AKKAYA B.

POLYMERS & POLYMER COMPOSITES, cilt.29, sa.9_suppl, 2021 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 29 Sayı: 9_suppl
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1177/09673911211035068
  • Dergi Adı: POLYMERS & POLYMER COMPOSITES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Chemical Abstracts Core, Chimica, Communication Abstracts, Compendex, INSPEC, Metadex, Civil Engineering Abstracts
  • Anahtar Kelimeler: antimicrobial, chitosan oligomer, cytotoxicity, heparin, metastasis, ANTIBACTERIAL ACTIVITY, ANTICOAGULANT ACTIVITY, DERIVATIVES, CHITOOLIGOSACCHARIDES, CHITIN, DRUG, INHIBITION, MECHANISMS, DELIVERY
  • Sivas Cumhuriyet Üniversitesi Adresli: Evet

Özet

In the present study, chitosan oligomer was modified to sulfated chitosan oligomer (ShCsO) to mimic heparin. Its chemical structure was determined by infrared spectroscopy (FT-IR), X-ray diffractometry (XRD), differential scanning calorimetry (DSC), and thermogravimetric analysis. The results showed that the FT-IR spectrum band at 799 cm(-1) corresponds to C-O-S and that at 1212 cm(-1) corresponds to S=O bond stretching, which prove that the sulfate groups are incorporated into chitosan oligomer successfully. The antimicrobial activity of ShCsO against to Bacillus subtilis in 1% concentration was 89.1 +/- 1.7%. The IC50 (mu g/ml) of ShCsO was 67.75, 56.07, 85.47, and 84.68 for A2780, MCF-7, DU-145, and HepG2, respectively. The results show that this newly synthesized material is a potential candidate to heparin-like chitosan derivatives. According to the literature, it was the first time that chitosan oligomer was modified to mimic heparin.