Magnetic deep eutectic solvents for vortex-assisted liquid phase microextraction method for selective and green extraction of patulin from fruits and fruit juices

Haq H. U., Boczkaj G., ALTUNAY N.

Microchemical Journal, cilt.208, 2025 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 208
  • Basım Tarihi: 2025
  • Doi Numarası: 10.1016/j.microc.2024.112472
  • Dergi Adı: Microchemical Journal
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, CAB Abstracts, Chemical Abstracts Core, Chimica, Food Science & Technology Abstracts, Index Islamicus, Veterinary Science Database
  • Anahtar Kelimeler: Box-Behnken design, Green solvents, Microextraction, Patulin analysis, UV–visible spectrophotometry
  • Sivas Cumhuriyet Üniversitesi Adresli: Evet

Özet

In this study, a vortex assisted magnetic deep eutectic solvent based liquid phase microextraction (VA-MDES-LPME) technique was developed for the extraction of patulin prior to its determination by ultraviolet–visible spectrophotometry (UV–Vis). For this purpose, the use of a magnetic deep eutectic solvent (MDES) consisting of trihexyltetradecylphosphonium chloride, MnCl2, and octanoic acid (molar ratio 1:2:2) was tested to perform an efficient extraction. Important experimental parameters (MDES-3 vol, pH, vortex time, and ethanol volume) were optimized using Box-Behnken Design. Under optimum conditions (MDES-3 vol 560 µL, pH 3.5, vortex time 300 s, and ethanol volume 300 µL), the limits of detection for the model solution and matrix-matched solution were 0.06 ng mL−1 and 0.37 ng mL−1, and the calibration ranges were 0.2–400 ng mL−1 and 1.2–300 ng mL−1, respectively. Furthermore, the extraction recovery was calculated to be 96.3–97.4 % with a low relative standard deviation (2.1–2.5 %). The VA-MDES-LPME technique was successfully applied for the determination of patulin from fruit and juice samples with an enrichment factor between169 and 185.