The aim of this in-vitro study was to evaluate microtensile bond strength (mu TBS) of two different self-adhesive composites (SACs) on the permanent dentin by applying five different universal adhesive systems. In this study, two different SACs [Vertise Flow (VF), Fusio Liquid Dentin (FLD)] and five different bonding systems [Clearfil Universal Bond Quick (CUB), Single Bond Universal (SBU), All Bond Universal (ABU), Prime Bond Universal (PBU), Futurabond U (FBU)] were used. A total of 22 groups were created in which SACs were applied without adhesive and with five different universal bonding agents in total-etch (TE) and self-etch (SE) modes. Two hundred and forty test sticks were obtained using 48 healthy human molar teeth in total with groups having 10 samples each. The mu TBS test was applied to each sample in the Universal test device and the data obtained were analyzed statistically by variance analysis and Tukey HSD test. In addition, the resin-dentin interface and fractures modes in the groups were examined by SEM. Upon examining the mu TBS results, the highest values were seen in the use of SBU adhesive in TE mode in VF group, while the lowest values were seen in the FLD control group. The difference between the control and experimental groups was found statistically significant (p < .05). Upon comparing the control groups with each other, it was seen that VF group had higher mu TBS values than FLD group and the difference between the groups was found statistically significant (p < .05). The mu TBS results and SEM images of the study showed that the use of SACs with universal adhesive systems provides a more effective bond strength.