Concanavalin a immobilized magnetic poly(glycidyl methacrylate) beads for antibody purification


AKKAYA B. , Yavuz H., CANDAN F. , DENİZLİ A.

JOURNAL OF APPLIED POLYMER SCIENCE, cilt.125, ss.1867-1874, 2012 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 125 Konu: 3
  • Basım Tarihi: 2012
  • Doi Numarası: 10.1002/app.34443
  • Dergi Adı: JOURNAL OF APPLIED POLYMER SCIENCE
  • Sayfa Sayıları: ss.1867-1874

Özet

Concanavalin A (Con A) immobilized magnetic poly(glycidyl methacrylate) (mPGMA) beads in monosize and spherical for (1.62 mu m in diameter) were used for the purification of human immunoglobulin G (IgG) from human plasma. Con A was immobilized by covalent binding onto the mPGMA beads. The maximum IgG adsorption on the mPGMA-Con A beads was observed at pH 6.0. The nonspecific IgG adsorption onto the plain mPGMA beads was very low (0.22 mg/g). Scatchard analysis of the adsorption isotherm for IgG on mPGMA-Con A beads showed an affinity constant (Ka) of 1.39 x 105 M-1 and a theoretical maximum adsorption capacity of 109.1 mg/g. An apparent IgG adsorption capacity of 66.2 mg/g was observed under the experimental conditions. IgG adsorption capacity from human plasma was observed as 48.0 mg/g. The adsorption of human serum albumin from plasma was 2.0 mg/g. The total protein adsorption was determined to be 50.0 mg/g. IgG molecules could be repeatedly adsorbed and eluted with the mPGMA-Con A beads without noticeable loss in the IgG adsorption capacity. (c) 2012 Wiley Periodicals, Inc. J Appl Polym Sci, 2012