An ellipsometric biosensor using aptamer for the detection of mercuric ions

Ertan T., ÇAĞLAYAN M. O.

CHEMICAL PAPERS, cilt.75, sa.1, ss.89-97, 2021 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 75 Sayı: 1
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1007/s11696-020-01282-3
  • Dergi Adı: CHEMICAL PAPERS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Chemical Abstracts Core
  • Sayfa Sayıları: ss.89-97
  • Anahtar Kelimeler: Biosensor, DNA aptamer, Ellipsometry, Mercuric ion
  • Sivas Cumhuriyet Üniversitesi Adresli: Evet

Özet

An ellipsometric biosensor, to be used in the detection of mercuric ions that affect the vital organs of living organisms, was developed. In this context, as a diagnostic element of the developed ellipsometric biosensor, a nucleic acid-based aptamer that is highly selective for mercuric ions (Hg2+) was used. Two different aptamers, a long and straight sequence, and a hairpin-structured oligonucleotide sequence were chosen. The detection sensitivity obtained by both aptamers was 0.237 degrees/mu M and 0.211 degrees/mu M, respectively. The detection limit was 0.89 pM Hg2+ and 187 nM Hg2+ for linear- and hairpin-type oligonucleotide, respectively.