Akademik Veri Yönetim Sistemi
3rd International Eurasian Conference on Biological and Chemical Sciences, Ankara, Türkiye, 19 - 20 Mart 2020, cilt.1, sa.1, ss.336
Several results have suggested that proton pump inhibitors have positive effects on the nervous system. Pantoprazole is one
of the proton pump inhibitors and widely used. The aim of this study was to investigate the effect of pantoprazole on
epileptic seizures and interaction with brain-derived neurotrophic factor (BDNF) in this effect. In this study, 18 male Wistar
Albino rats were used. Animals divided into three groups as control, saline (serum physiologic 1 ml/kg for 7 days) and
pantoprazole (10 mg/kg for 7 days). Seventh days after 30 min the administration of the last drug, pentylenetetrazole (PTZ;
45 mg/kg) was given to saline and pantoprazole groups to induced seizures. The animals were observed for 30 min. Seizure
stages (according to the Racine Scale) and first myoclonic jerk times (FMJ). After 24 hours of PTZ injection, brain tissues
of all groups animal were removed. Cortex and hippocampus separated and homogenized. BDNF was measured by using
the sandwich-ELISA method in the homogenates. Statistical evaluation of the data was performed by one-way ANOVA and
multiple comparisons were determined by the Tukey test. Statistical significance was defined at p<0.05. Obtained data
suggest that pantoprazole decreases seizure stages and increases FMJ compared to saline group (p<0,05). On the other hand,
there is no statistical significance on BDNF levels in the cortex between groups (p>0.05). However, there is increasing in
BDNF levels in the hippocampus for pantoprazole group compared to saline (p<0,05). In conclusion, this study suggests
that pantoprazole increases BDNF level in hippocampus after PTZ-induced seizures.
Keywords: Epilepsy, seizures, pentylenetetrazole, pantoprazole, brain-derived neurotrophic factor, rat