In this study, anatomical and palynological properties of Chaerophyllum astrantiae Boiss. & Bal. and C. aureum L. were determined for the first time. Cross sections of roots, stems, stem leaves and fruits of both species were examined. The leaves are amphistomatic and mesophyll is bifacial The fruit has two vittae on commissural face and four vittae on lateral and dorsal faces in both species. The palynological studies revealed that, C. astrantiae and C. aureum have tricolporate pollen type and perforate exine ornamentation under light microscope. Ratio of polar axis to equatorial axis is 2.15 in C. astrantiae and 2.05 in C. aureum. Thus, pollen shape is perprolate for both of the species.