Biosynthesized silver nanoparticles and miR34a mimics mediated activation of death receptor in MCF-7 human breast cancer cell lines

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Iqbal M. J., Rashid U., Javed Z., Hamid Z., Imran K., Kabeer A., ...Daha Fazla

CANCER NANOTECHNOLOGY, cilt.13, sa.1, 2022 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 13 Sayı: 1
  • Basım Tarihi: 2022
  • Doi Numarası: 10.1186/s12645-022-00137-8
  • Dergi Adı: CANCER NANOTECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, EMBASE, Directory of Open Access Journals
  • Anahtar Kelimeler: MCF-7 cell lines, Silver nanoparticles synthesis, DR4, DR5, Breast cancer, EXTRACT
  • Sivas Cumhuriyet Üniversitesi Adresli: Evet

Özet

Nano-biotechnology-based clinical applications to cure health-related issues have gained huge attention among the scientific community and hold great promise to limit cancer metastasis. In this study, green-derived silver nanoparticles were synthesized by using leaf extract of Litchi chinensis. Characterization of biosynthesized silver nanoparticles was performed by using UV-Vis spectroscopy, FTIR, XRD, EDS, and SEM analysis. The clinical application of green-drive nanoparticles was investigated by using MCF-7 cancer cell lines. MCF-7 breast cancer cell lines were analyzed against three different treatments. (i) Silver nanoparticles (AgNPs), (ii) miR34a mimics and (iii) Co-delivery of AgNPs and miR34a mimics. Cell viability was determined by MTT assay and, extraction of mRNA and cDNA synthesis were performed after successful cellular transfection. qRT-PCR was done for expression analysis of DR4 and DR5 upon exogenous delivery of all 3 treatments. Results indicate that L. chinensis leaves have a significant amount of phenolic and flavonoid contents and also possess massive antioxidant activity. The diameter of nanoparticles was observed in the range of 41-55 nm. It was concluded that green-derived silver nanoparticles can be a potential contributing agent for cancer prevention and are reported to upregulate the expression of DR4 and DR5 by 0.8-folds and 3.7-folds, respectively.