Akademik Veri Yönetim Sistemi
15. Ulusal / 1. Uluslararası Histoloji ve Embriyoloji Kongresi, 26 - 28 Mayıs 2022, ss.114-115
Introduction: Implantation in mammals is one of the complex biological events that occur as a result of the dialogue between the
blastocyst stage embryo and the receptive endometrium. Ang-1 (Angiopoietin-1), ET-1 (Endothelin-1), and Cdh-5 (VE-Cadherin)
proteins play a critical role in mediating the interaction between the endothelium and surrounding matrix and mesenchyme in vascular proliferation during embryo invasion.
Objective: We aimed to investigate the possible roles above mentioned proteins in angiogenesis by determining their immunolocalizations in the estrus cycle and gestational days in rat endometrium.
Material-Methods: Immunofluorescence staining was applied to sections taken from uterine tissues. In addition, the mRNA expressions of the above-mentioned proteins were determined by the Real-Time PCR method from the uterine endometrium.
Results: Cdh5 was found to have very strong immunolocalization especially in proestrus, estrus and metaestrus stages; ET-1, on the other hand, showed very strong immunostaining in the endothelium of endometrial blood vessels in diestrus stage. It was determined that all 3 proteins had relatively weak immunostaining in the luminal and glandular epithelia during all cycle stages. Ang-1 was detected to have very strong reaction in the cytoplasm of stromal cells, especially in the proestrus and diestrus stages. At days 7.5 and 8.5 of gestation, ET-1 and Cdh5 showed strong/very strong staining in the endothelium of blood vessels in the mesometrial area. Immunolocalization of Ang-1 was relatively strong in the cytoplasm of undifferentiated stromal cells.
Real-Time PCR analyzes demonstrated that ET-1 gene expression was upregulated (8.87-fold, 55.45%) in the transition from proestrus to estrus, while Cdh-5 and Ang-1 gene expressions were downregulated by 0.36-fold (15.8%) and 0.52-fold (31.2%) respectively. When transitioning from the diestrus to proestrus, the inverse relationship between the ET-1 and Cdh-5 genes was disrupted and the expressions of both genes were downregulated. When the gestational days were examined, the inverse relationship between Cdh-5 and Ang-1 was remarkable. Although ET-1 gene expression decreased relatively from the days 8.5 to the 9.5 of pregnancy, it was upregulated during all days of pregnancy. Accordingly, it was determined that there was no correlation between genes, and each gene exhibited different patterns in terms of gestational days and cycle stages.
Conclusion: It supports the idea that the studied angiogenetic factor proteins may be important modulators in the changes in the oestrus cycle and angiogenic events occurring in embryo implantation, and may play important roles in critical functions such as implantation, decidualization and placentation in the human endometrium.