Gallic acid improves the viability and mitochondrial membrane potential of post-thawed goat buck semen

Inanc M. E., Gungor S., Gul E., Uslu B. A., Ata A.

ACTA VETERINARIA HUNGARICA, cilt.69, sa.3, ss.291-297, 2021 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 69 Sayı: 3
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1556/004.2021.00038
  • Dergi Adı: ACTA VETERINARIA HUNGARICA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, EMBASE, MEDLINE, Veterinary Science Database
  • Sayfa Sayıları: ss.291-297
  • Anahtar Kelimeler: gallic acid, frozen-thawed, goat semen, flow cytometry, PLASMA-MEMBRANE, BULL SEMEN, INTEGRITY, CRYOPRESERVATION, ANTIOXIDANT, SPERMATOZOA, TREHALOSE, APOPTOSIS, TOXICITY, PROTECTS
  • Sivas Cumhuriyet Üniversitesi Adresli: Evet

Özet

The aim of this study was to determine the effects of gallic acid (GA) on frozen-thawed goat spermatozoa. Four Honamli goat bucks were used at their breeding season, and ejaculates were collected by an electroejaculator. Mixed semen was divided into the following four groups: control (0 mM), low (L; 1 mM), medium (M; 2 mM), and high (H; 4 mM) concentration of GA. All the groups were frozen and thawed in a water bath for spermatological evaluation. The lowest motility was observed in the control group (47.60 +/- 5.70%) (P < 0.05), while the highest viability (62.45 +/- 1.68%), plasma membrane and acrosome integrity (44.81 +/- 4.57%), and high mitochondrial membrane potential (35.96 +/- 2.50%) were observed in the low GA group (P < 0.05). Also, the lowest hypo-osmotic swelling test (HOS +) value was found in the high GA group (47.60 +/- 4.82%) (P < 0.05). In conclusion, supplementing a low concentration (1 mM) of GA to the Tris-based semen extender had a positive effect on spermatological parameters after freeze-thawing of Honamli goat semen. Further studies should be continued in other species with different doses and combinations using commercial and/or homemade semen extenders.