A preconcentration method for indirect determination of acrylamide from chips, crackers and cereal-based baby foods using flame atomic absorption spectrometry


TALANTA, vol.161, pp.143-150, 2016 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 161
  • Publication Date: 2016
  • Doi Number: 10.1016/j.talanta.2016.08.053
  • Title of Journal : TALANTA
  • Page Numbers: pp.143-150


Acrylamide is a toxic species for human health, and is a Maillard reaction product which forms spontaneously in heat treatment process of foods. Therefore, a simple, fast and cost-effective method was developed for the indirect determination of acrylamide in processed foods particularly consumed by children. The method is based on ion-pairing of acrylamide with fluorescein (F2-) in presence of Ni(II) ions at pH 9.0, and then extraction of the formed ternary complex into micellar phase of poly(ethyle-neglycol-mono-p-nonylphenylether) (PONPE 7.5) before analysis by flame atomic absorption spectrometry (FAAS). The ultrasonic-assisted cloud point extraction (UA-CPE) has been used for the pre concentration of acrylamide in the samples prior to its FMS detection. The matrix matched calibration curve is linear in range of 0.3-150 mu g kg(-1) under optimal reagent conditions (1.75 mL of 0.1 mol L-1 ammonia buffer at pH 9.0, 2.2 mg L-1 Ni(II), 4.0 x 10(-4) mol L-1 F2-, 0.4% (w/v) NH4Cl and 0.7% (v/v) PONPE 7.5) with sensitivity enhancement of 160-fold. The proposed method has been validated by assessment of the following parameters; the limits of detection (LOD) and quantification (LOQ) (0.08 mu g kg(-1) and 0.28 mu g kg(-1), respectively) with a relative standard deviation (RSD%) lower than 6.3%, and extractive recovery higher than 95% for acrylamide spiked at levels of 5 and 25 mu g kg(-1). The method was successfully applied to the indirect determination of acrylamide in the processed foods and two CRMs with satisfactory results. (C) 2016 Elsevier B.V. All rights reserved.