The determination of inorganic arsenic species in water, beverages and foods become crucial in recent years, because arsenic species are considered carcinogenic and found at high concentrations in the samples. This communication describes a new cloud-point extraction (CPE) method for the determination of low quantity of arsenic species in the samples, purchased from the local market by UV-Visible Spectrophotometer (UV-Vis). The method is based on selective ternary complex of As(V) with acridine orange (AOH(+)) being a versatile fluorescence cationic dye in presence of tartaric acid and polyethylene glycol tert-octylphenyl ether (Triton X-114) at pH 5.0. Under the optimized conditions, a preconcentration factor of 65 and detection limit (3S(blank)/m) of 1.14 mu g L-1 was obtained from the calibration curve constructed in the range of 4-450 mu g L-1 with a correlation coefficient of 0.9932 for As(V). The method is validated by the analysis of certified reference materials (CRMs). (C) 2015 Elsevier Ltd. All rights reserved.