A Novel Preconcentration Procedure Using Neutral Red as Ion-Pairing Reagent for Determination of Inorganic Dissolved Arsenic Species in Different Water and Beverages by Spectrophotometry

Guerkan R. , Kir U., ALTUNAY N.

FOOD ANALYTICAL METHODS, vol.8, no.7, pp.1637-1651, 2015 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 8 Issue: 7
  • Publication Date: 2015
  • Doi Number: 10.1007/s12161-014-0039-9
  • Title of Journal : FOOD ANALYTICAL METHODS
  • Page Numbers: pp.1637-1651


In the current study, a new cloud point extraction (CPE) procedure was developed for the separation and preconcentration of inorganic soluble As species, As(III), As(V), and total As in water and beverage samples. Selective ion-pairing complex of As(III) with Neutral red (NRH+) being a cationic phenazine-based dye in presence of citric acid at pH 2.0 was extracted into the surfactant-rich phase of octylphenoxypolyethoxyethanol (Triton X-114) from samples. After phase separation, the preconcentrated As(III) was determined by means of spectrophotometer at 542 nm. After optimization of the CPE conditions, a preconcentration factor of 50 and the detection and quantification limits of 1.44 and 4.8 mu g L-1 with a correlation coefficient of 0.9953 were obtained from the calibration curve constructed in the range of 5-1500 mu g L-1 for As(III). The precision of the method (as RSD) was in the range of 2.2-4.5 % (25, 100, and 750 mu g L-1, N = 5). The As(V) contents of samples were calculated from the difference between As(III) and total As contents after the reduction of As(V) to As(III) with mixture of KI and ascorbic acid at HCl media. The method is very versatile and inexpensive because it exclusively used conventional UV-Vis spectrophotometry. The method was succesfully applied to the simultenous determination of inorganic arsenic species in different water and beverage samples. Its accuracy and precision were controlled by analysis of two certified reference materials (CRMs).